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KMID : 0895420090190030261
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Journal of Korean Society of Occupational and Enviromental Hygiene
2009 Volume.19 No. 3 p.261 ~ p.269
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Effect of Superoxide Dismutase on Oxidative Stress of Reactive Oxygen Species in Cultured Human Skin Melanocyte
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Seo Young-Mi
Kim Nam-Song
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Abstract
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To evaluate the effect of antioxidant on the cytotoxicity induced by oxidative stress of reactive oxygen species (ROS) in cultured human skin melanocytes, colorimeric assay of XTT and tyrosinase activity assay were adopted after human skin melanocytes were preincubated for 2 hours in the media containing various concentrations of superoxide dismutase(SOD) before the treatment of hydrogen peroxide. Light microscopic study was carried out in same cultures. The results of this study were as follows 1. Cell viability of human skin melanocytes was significantly decreased by 30 and 40 μM of hydrogen peroxide(H2O2), respectively. 2. XTT50 was determined at 30 μM after human skin melanocytes were treated with 10~40 μM of hydrogen peroxide for 6 hours. 3. The cell viability of cultured human skin melanocytes pretreated with SOD was increased than that of cultured human skin melanocytes treated with H2O2 dose-dependently. 4. In tyrosinase activity of human skin melanocytes, the cell treated with SOD showed brown stain compared with H2O2 treated cells, dark stain. 5. In light microscopy, cultured human skin melanocytes exposed to H2O2 showed morphological changes such as the decreased cell number and cytoplasmic processes, compared with control. 6. In light microscopy, cultured human skin melanocytes pretreated with SOD showed the increase of cell number and cytoplasmic processes compared with H2O2-treated group. From these results, it is suggested that oxidative stress of ROS such as H2O2 has cytotoxicity by showing the decreased cell viability, the increased tyrosinase activity and mophological changes of the decreased cell number and cytoplasmic processes. While, antioxidant like SOD was effective in the prevention of oxidative stress-mediated cytotoxicity by the increased cell viability, decreased tyrosinase activity and the protection of degenerative morphological changes in cultured human skin melanocytes.
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KEYWORD
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Superoxide Dismutase, Oxidative Stress, Melanocyte
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